Fluorescence Software | Vinci

Vinci

Overview
Specifications
Measurement Examples

Vinci ••••
A comprehensive Multidimensional Fluorescence Spectroscopy software program designed to enhance the capabilities and performance of ISS spectrofluorometers.

Vinci offers unparalled performance to the end-user through three distinct features:

Instrument Control
Vinci provides full control over all automated instrument components, including shutters, polarizers, sample holders, monochromators, and enables integration of external devices such as stopped-flow apparatus, titrator, and temperature bath. Vinci's remote interface allows ISS Support Personnel to access any instrument in the world via internet and conduct a remote diagnostic check-up.

Data Acquisition
Vinci offers a variety of data acquisition options (spectra, lifetime, kinetics, titrations) through built-in, easy-to-use, routines and allows the user to generate, store and rerun custom-designed data acquisition protocols. Data are stored in ASCII format for convenient access from other software packages.

Data Analysis
Vinci's fast fitting routines allow to retrieve multiple decay times and multiple rotational correlation times, to obtain time-resolved spectra with picosecond resolution and to separate steady-state spectra of up to three components in a mixture using the phase- and modulation-resolved spectra measurement option. Other operations include smoothing, derivative, calculation of area and arithmetic between files. A superb graphical interface generates and displays 2D and 3D plots that can be exported in internet- and presentation-ready formats. A report can be saved in Word or PDF format.

Instrument Control

Automation of ISS instrument components Control of external devices Temperature bath (Neslab, Fisher) Titrator (ISS, Hamilton) Stopped-flow apparatus (Molecular Kinetics, OLIS, Hi-Tech, Applied Photophysics, Kintek) Pressure pump Recording from temperature/pressure sensors Storage of all experimental parameters in the experiment data file

Automation of ISS instrument components
Control of external devices
- Temperature bath (Neslab, Fisher)
- Titrator (ISS, Hamilton)
- Stopped-flow apparatus (Molecular Kinetics, OLIS, Hi-Tech, Applied Photophysics, Kintek)
- Pressure pump
Recording from temperature/pressure sensors
Storage of all experimental parameters in the experiment data file

Data Acquisition

One-dimensional measurements
- Corrected Excitations and emission spectra
- Excitation-Emission matrices
- Polarization/Anisotropy
- Kinetics (slow, fast)
- Titrations
- Fluorescence decay times (exponential and non-exponential)
- Rotational correlation times (isotropic, anisotropic, hindered)
- Time-resolved spectra
- Phase- and modulation-resolved spectra

Multi-dimensional measurements

The user can select from any of the following experimental combinations:

Fluorescence Intensity Polarization/Anisotropy Ratiometric Measurements	vs.	Excitation (Emission) Wavelength and/or any of the following: Emission (Excitation) Wavelength Sample Concentration Sample Temperature Sample Pressure
Fluorescence Intensity Polarization/Anisotropy Ratiometric Measurements	vs.	Time and/or any of the following: Excitation Wavelength Emission Wavelength Sample Concentration Sample Temperature Sample Pressure
Phase and Modulation Differential Polarization	vs.	Modulation Frequency and/or any of the following: Excitation Wavelength Emission Wavelength Sample Concentration Sample Temperature Sample Pressure

User defined protocols
The user can define protocols for both one-dimensional and multi-dimensional measurements. Protocols can be stored and rerun.

Data Analysis

Spectral Analysis	Normalization Smoothing (average, median, Savitzky-Golay) Derivative (nth-order) Integration (area, average and standard deviation) Spectral correction Spectral moments Conversion to wave numbers Data Interpolation User-defined filtering Fitting to user-defined model
Spectral Operations	Addition, subtraction, multiplication, division Scaling factor
Time-Resolved Analysis	Multiple decays for up to 4 components for exponential, non exponential and lifetime distributions Rotational correlation times for isotropic, anisotropic and hindered rotators User-defined models Phase- and modulation-resolved spectra Time-resolved spectra
Simulation	Phase and demodulation versus modulation frequency for up to 4 components for exponential, non exponential and lifetime distributions Differential phase and modulation ration versus modulation frequency for isotropic, anisotropic and hindered rotators
Display	2D and 3D display with user-defined colors and fonts 3D surface rotation and in/out zooming Cursor identification of XY spectra coordinates
Data Format	The data files are stored in an accessible text (ASCII) format. The experiment data file, or raw data file, includes the information about the experiment protocol and, as such, it can be re-loaded to rerun the original experiment. Experiment data files cannot be overwritten; once an experiment data file is modified, the new file is stored as analysis data file. Experiment files and Analysis files are identifiable through their different extensions and icons.
Data and Plots Export	Plots export to gif, tiff, jpeg, png, bitmaps and metafiles Compatible with LFD and CFS formats
Links	To Global Analysis by Globals Unlimited To DATAN by MultiD Analysis AB
Import	ISS (cat, lif, pol), LFD, SLM
Operating System Supported	Windows 10

Frequency responses (phase and modulation) of 2-Aminopurine in water acquired on K2™ using a Xenon lamp. The excitation wavelength was 290-nm with a 2 mm slit size. The emission was collected through a 345 high pass filter. The data is best fitted with a single exponential decay time of 11.69 ns (χ² = 1.32).

Frequency-domain anisotropy decays (Differential Polarized Phase Angle and Amplitude Ratio) of Coumarin 6 in ethylene glycol measured on ChronosFD™ using a 473-nm laser diode. The emission was collected using a 500-nm long-pass filter. Calculated values for θ = 2.0 ns with R₀ = 0.38 and τ = 2.33 ns, T = 27-28°C.